RAxML-NG v. 1.0.2 released on 22.02.2021 by The Exelixis Lab. Developed by: Alexey M. Kozlov and Alexandros Stamatakis. Contributors: Diego Darriba, Tomas Flouri, Benoit Morel, Sarah Lutteropp, Ben Bettisworth. Latest version: https://github.com/amkozlov/raxml-ng Questions/problems/suggestions? Please visit: https://groups.google.com/forum/#!forum/raxml System: Intel(R) Xeon(R) CPU E5-2690 v4 @ 2.60GHz, 28 cores, 251 GB RAM RAxML-NG was called at 12-Jul-2021 17:29:01 as follows: raxml-ng --ancestral --msa /truba/home/emrah/WORKFOLDER/PROD/300621_run/phylogeny-snakemake/results/Q9BSJ1/2_msa/Q9BSJ1_nogap_msa.fasta --tree /truba/home/emrah/WORKFOLDER/PROD/300621_run/phylogeny-snakemake/results/Q9BSJ1/3_mltree/Q9BSJ1.raxml.bestTree_unrooted --model LG4X --prefix /truba/home/emrah/WORKFOLDER/PROD/300621_run/phylogeny-snakemake/results/Q9BSJ1/4_raxmlng_ancestral/Q9BSJ1 --threads 4 Analysis options: run mode: Ancestral state reconstruction start tree(s): user random seed: 1626100141 tip-inner: ON pattern compression: OFF per-rate scalers: OFF site repeats: OFF branch lengths: proportional (ML estimate, algorithm: NR-FAST) SIMD kernels: AVX2 parallelization: coarse-grained (auto), PTHREADS (4 threads), thread pinning: OFF [00:00:00] Reading alignment from file: /truba/home/emrah/WORKFOLDER/PROD/300621_run/phylogeny-snakemake/results/Q9BSJ1/2_msa/Q9BSJ1_nogap_msa.fasta [00:00:00] Loaded alignment with 1001 taxa and 452 sites WARNING: Sequences tr_M3YK97_M3YK97_MUSPF_9669 and tr_G1M498_G1M498_AILME_9646 are exactly identical! WARNING: Sequences tr_M3YK97_M3YK97_MUSPF_9669 and tr_A0A2U3ZPJ8_A0A2U3ZPJ8_ODORO_9708 are exactly identical! WARNING: Sequences tr_M3YK97_M3YK97_MUSPF_9669 and tr_A0A2U3XSM4_A0A2U3XSM4_LEPWE_9713 are exactly identical! WARNING: Sequences tr_M3YK97_M3YK97_MUSPF_9669 and tr_A0A2Y9JZW9_A0A2Y9JZW9_ENHLU_391180 are exactly identical! WARNING: Sequences tr_M3YK97_M3YK97_MUSPF_9669 and tr_A0A384C9P1_A0A384C9P1_URSMA_29073 are exactly identical! WARNING: Sequences tr_G1RI87_G1RI87_NOMLE_61853 and tr_M3ZBJ4_M3ZBJ4_NOMLE_61853 are exactly identical! WARNING: Sequences tr_H2PIE3_H2PIE3_PONAB_9601 and tr_A0A0D9R725_A0A0D9R725_CHLSB_60711 are exactly identical! WARNING: Sequences tr_F1P646_F1P646_CANLF_9615 and tr_I7FTT9_I7FTT9_PIG_9823 are exactly identical! WARNING: Sequences tr_A0A2J8ITD5_A0A2J8ITD5_PANTR_9598 and tr_A0A2R8ZI57_A0A2R8ZI57_PANPA_9597 are exactly identical! WARNING: Sequences tr_H2Q2Y1_H2Q2Y1_PANTR_9598 and sp_P19474_RO52_HUMAN_9606 are exactly identical! WARNING: Sequences tr_H2QSH5_H2QSH5_PANTR_9598 and sp_O00478_BT3A3_HUMAN_9606 are exactly identical! WARNING: Sequences tr_H2QSH7_H2QSH7_PANTR_9598 and tr_A0A2R9BIQ5_A0A2R9BIQ5_PANPA_9597 are exactly identical! WARNING: Sequences tr_H2R534_H2R534_PANTR_9598 and tr_A0A2R8Z7C3_A0A2R8Z7C3_PANPA_9597 are exactly identical! WARNING: Sequences tr_K7BVH2_K7BVH2_PANTR_9598 and sp_Q96F44_TRI11_HUMAN_9606 are exactly identical! WARNING: Sequences tr_K7BVH2_K7BVH2_PANTR_9598 and tr_A0A2R8ZKT7_A0A2R8ZKT7_PANPA_9597 are exactly identical! WARNING: Sequences tr_A0A1D5QTX6_A0A1D5QTX6_MACMU_9544 and tr_A0A2K5M5M7_A0A2K5M5M7_CERAT_9531 are exactly identical! WARNING: Sequences tr_A0A1D5REF7_A0A1D5REF7_MACMU_9544 and tr_A0A2K6CBM5_A0A2K6CBM5_MACNE_9545 are exactly identical! WARNING: Sequences tr_F6RTU9_F6RTU9_MACMU_9544 and tr_A0A2K6B5B4_A0A2K6B5B4_MACNE_9545 are exactly identical! WARNING: Sequences tr_F6U9E0_F6U9E0_MACMU_9544 and tr_G7P3M3_G7P3M3_MACFA_9541 are exactly identical! WARNING: Sequences tr_F6W0V1_F6W0V1_MACMU_9544 and tr_G7P6Q0_G7P6Q0_MACFA_9541 are exactly identical! WARNING: Sequences tr_F6W0V1_F6W0V1_MACMU_9544 and tr_A0A2K5YPH2_A0A2K5YPH2_MANLE_9568 are exactly identical! WARNING: Sequences tr_F7C1A0_F7C1A0_MACMU_9544 and tr_A0A2K6B3T4_A0A2K6B3T4_MACNE_9545 are exactly identical! WARNING: Sequences tr_F7EGV7_F7EGV7_MACMU_9544 and tr_A0A2K5MH78_A0A2K5MH78_CERAT_9531 are exactly identical! WARNING: Sequences tr_F7GJW9_F7GJW9_MACMU_9544 and tr_A0A2K6BDB8_A0A2K6BDB8_MACNE_9545 are exactly identical! WARNING: Sequences tr_F7HRB7_F7HRB7_MACMU_9544 and tr_A0A2K6DW68_A0A2K6DW68_MACNE_9545 are exactly identical! WARNING: Sequences tr_G7P2P4_G7P2P4_MACFA_9541 and tr_A0A2K6EB40_A0A2K6EB40_MACNE_9545 are exactly identical! WARNING: Sequences tr_A0A096N7V8_A0A096N7V8_PAPAN_9555 and tr_A0A2K5MWE8_A0A2K5MWE8_CERAT_9531 are exactly identical! WARNING: Sequences tr_A0A096NAM1_A0A096NAM1_PAPAN_9555 and tr_A0A2K5LKX8_A0A2K5LKX8_CERAT_9531 are exactly identical! WARNING: Sequences tr_A0A096NAM1_A0A096NAM1_PAPAN_9555 and tr_A0A2K5YAG4_A0A2K5YAG4_MANLE_9568 are exactly identical! WARNING: Sequences tr_A0A091JG56_A0A091JG56_EGRGA_188379 and tr_A0A091UZ29_A0A091UZ29_NIPNI_128390 are exactly identical! WARNING: Sequences tr_A0A091V004_A0A091V004_NIPNI_128390 and tr_A0A087R134_A0A087R134_APTFO_9233 are exactly identical! WARNING: Sequences tr_A0A091V0M9_A0A091V0M9_NIPNI_128390 and tr_A0A087R149_A0A087R149_APTFO_9233 are exactly identical! WARNING: Sequences tr_A0A091V6Y1_A0A091V6Y1_OPIHO_30419 and tr_A0A091GQX6_A0A091GQX6_9AVES_55661 are exactly identical! WARNING: Sequences tr_A0A091V6Y1_A0A091V6Y1_OPIHO_30419 and tr_A0A0A0AK23_A0A0A0AK23_CHAVO_50402 are exactly identical! WARNING: Sequences tr_A0A2Y9MXG3_A0A2Y9MXG3_DELLE_9749 and tr_A0A2Y9EPM4_A0A2Y9EPM4_PHYCD_9755 are exactly identical! WARNING: Sequences tr_A0A2Y9MXG3_A0A2Y9MXG3_DELLE_9749 and tr_A0A384B2Y7_A0A384B2Y7_BALAS_310752 are exactly identical! WARNING: Duplicate sequences found: 36 NOTE: Reduced alignment (with duplicates and gap-only sites/taxa removed) NOTE: was saved to: /truba/home/emrah/WORKFOLDER/PROD/300621_run/phylogeny-snakemake/results/Q9BSJ1/4_raxmlng_ancestral/Q9BSJ1.raxml.reduced.phy Alignment comprises 1 partitions and 452 sites Partition 0: noname Model: LG4X+R4 Alignment sites: 452 Gaps: 27.24 % Invariant sites: 0.00 % NOTE: Binary MSA file created: /truba/home/emrah/WORKFOLDER/PROD/300621_run/phylogeny-snakemake/results/Q9BSJ1/4_raxmlng_ancestral/Q9BSJ1.raxml.rba Parallelization scheme autoconfig: 1 worker(s) x 4 thread(s) Parallel reduction/worker buffer size: 1 KB / 0 KB [00:00:00] Loading user starting tree(s) from: /truba/home/emrah/WORKFOLDER/PROD/300621_run/phylogeny-snakemake/results/Q9BSJ1/3_mltree/Q9BSJ1.raxml.bestTree_unrooted [00:00:00] Data distribution: max. partitions/sites/weight per thread: 1 / 113 / 9040 [00:00:00] Data distribution: max. searches per worker: 1 Starting ML tree search with 1 distinct starting trees [00:00:00] Tree #1, initial LogLikelihood: -215546.374649 [00:00:00 -215546.374649] Initial branch length optimization [00:00:01 -213982.772069] Model parameter optimization (eps = 0.100000) [00:00:46] Tree #1, final logLikelihood: -213162.864012 Optimized model parameters: Partition 0: noname Rate heterogeneity: FREE (4 cats, mean), weights&rates: (0.084433,0.518907) (0.063270,0.765968) (0.416979,0.772036) (0.435318,1.345687) Base frequencies (model): M0: 0.147383 0.017579 0.058208 0.017707 0.026331 0.041582 0.017494 0.027859 0.011849 0.076971 0.147823 0.019535 0.037132 0.029940 0.008059 0.088179 0.089653 0.006477 0.032308 0.097931 M1: 0.063139 0.066357 0.011586 0.066571 0.010800 0.009276 0.053984 0.146986 0.034214 0.088822 0.098196 0.032390 0.021263 0.072697 0.016761 0.020711 0.020797 0.025463 0.045615 0.094372 M2: 0.062457 0.066826 0.049332 0.065270 0.006513 0.041231 0.058965 0.080852 0.028024 0.037024 0.075925 0.064131 0.019620 0.028710 0.104579 0.056388 0.062027 0.008241 0.033124 0.050760 M3: 0.106471 0.074171 0.044513 0.096390 0.002148 0.066733 0.158908 0.037625 0.020691 0.014608 0.028797 0.105352 0.007864 0.007477 0.083595 0.055726 0.047711 0.003975 0.010088 0.027159 Substitution rates (model): M 0: 0.295719 0.067388 0.253712 1.029289 0.107964 0.514644 10.868848 0.380498 0.084223 0.086976 0.188789 0.286389 0.155567 1.671061 2.132922 0.529591 0.115551 0.102453 0.916683 0.448317 0.457483 0.576016 1.741924 0.736017 0.704334 5.658311 0.123387 0.221777 93.433377 0.382175 0.235965 6.535048 0.525521 0.303537 0.641259 0.289466 0.102065 2.358429 0.251987 0.216561 0.503084 0.435271 4.873453 0.090748 0.033310 0.746537 0.128905 0.127321 0.904011 0.939733 0.435450 0.046646 0.262076 0.043986 0.189008 0.599450 109.901504 1.070052 5.229858 0.052764 0.021407 0.621146 0.081091 0.205164 5.164456 0.747330 0.308078 0.260889 0.185083 0.080708 0.029955 0.084794 1.862626 0.553477 0.151733 0.230320 0.096955 0.352526 0.590018 0.386853 1.559564 0.606648 0.587531 0.592318 0.885230 4.117654 0.246260 6.508329 0.054187 0.195703 1.669092 0.810168 0.066081 2.437439 0.165666 0.106333 0.093417 0.035149 0.072549 1.202023 1.634845 0.060194 0.069359 2.448827 0.232297 0.064822 3.537387 0.435384 0.290413 0.280695 0.105999 0.206603 0.404968 0.048984 0.069963 0.256662 0.228519 0.241077 4.320442 3.656545 0.290216 0.307466 0.096556 0.306067 0.204296 0.504221 1.991533 0.655465 6.799829 11.291065 0.961142 0.448965 6.227274 20.304886 0.205944 1.495537 0.091940 1.994320 0.754940 0.170343 0.050315 0.372166 0.206332 0.097050 5.381403 0.122332 3.256485 2.261319 0.848067 0.064441 0.102493 0.459041 0.133091 0.561215 0.457430 0.163849 5.260446 0.360946 0.389413 0.033291 0.115301 0.112593 1.559944 0.426508 0.132547 0.498634 0.559069 0.264728 0.693307 0.438856 0.306683 0.109129 18.392863 66.647302 0.400021 4.586081 2.099355 0.411347 0.476350 0.584622 3.634276 0.101797 0.148995 0.089177 0.034710 0.063603 0.755865 20.561407 0.133790 0.154902 M 1: 0.066142 0.590377 0.069930 9.850951 1.101363 0.150375 0.568586 0.051668 0.127170 0.292429 0.071458 1.218562 0.075144 7.169085 30.139501 13.461692 0.021372 0.045779 4.270235 0.468325 0.013688 0.302287 1.353957 0.028386 0.037750 0.262130 0.016923 0.064289 0.855973 0.079621 0.011169 0.161937 0.276530 0.161053 0.081472 0.036742 0.030342 2.851667 3.932151 8.159169 0.219934 0.421974 2.468752 0.344765 0.210724 1.172204 0.763553 0.082464 0.726566 11.149790 4.782635 0.058046 0.498072 0.258487 0.146882 0.249672 0.560142 0.046719 0.106259 0.003656 0.004200 0.014189 0.009876 0.002656 0.040244 0.267322 0.053740 0.006597 0.027639 0.012745 0.582670 0.005035 0.275844 0.098208 0.445038 1.217010 0.033969 1.988516 0.681161 0.825960 18.762977 11.949233 0.286794 0.534219 4.336817 3.054085 0.129551 4.210126 0.165753 1.088704 1.889645 3.344809 0.111063 2.067758 3.547017 2.466507 0.188236 0.203493 0.281953 0.037250 0.029788 0.008541 0.014768 0.125869 0.056702 0.004186 0.110993 0.201148 0.139705 0.009201 0.012095 0.043812 0.013513 0.002533 0.005848 0.031390 0.021612 0.004854 0.129497 0.976631 0.053397 0.019475 0.004964 0.015539 0.031779 0.064558 0.065585 0.079927 0.095591 0.196886 0.408834 0.126088 0.037226 0.452302 0.016212 7.278994 0.029917 7.918203 0.450964 0.169797 0.104288 1.578530 0.015909 0.094365 16.179952 0.042762 14.799537 1.506485 0.637893 0.123793 0.641351 0.154810 0.140750 3.416059 0.259400 0.009457 0.090576 0.292108 0.297913 0.017172 0.021976 0.032578 1.375871 0.457399 0.598048 4.418398 0.239749 0.168432 2.950318 0.143327 0.328689 0.125011 0.562720 1.414883 0.227807 3.478333 2.984862 0.061299 0.077470 1.050562 13.974326 0.154326 0.224675 0.112000 0.060703 0.123480 5.294490 0.447011 0.033381 0.045528 M 2: 0.733336 0.558955 0.503360 4.149599 1.415369 1.367574 1.263002 0.994098 0.517204 0.775054 0.763094 1.890137 0.540460 0.200122 4.972745 1.825593 0.450842 0.526135 3.839269 0.597671 0.058964 2.863355 2.872594 0.258365 0.366868 2.578946 0.358350 0.672023 5.349861 0.691594 0.063347 0.032875 0.821562 0.580847 0.661866 0.265730 0.395134 5.581680 1.279881 1.335650 0.397108 1.840061 5.739035 0.284730 0.109781 1.612642 0.466979 0.141582 0.019509 4.670980 1.967383 0.088064 0.581928 0.145401 0.225860 0.434096 2.292917 1.024707 0.821921 0.027824 0.021443 0.088850 0.060820 0.018288 0.042687 1.199607 0.420710 0.037642 0.141233 0.090101 1.043232 0.209978 0.823594 3.039380 1.463390 1.983693 0.397640 2.831098 4.102068 0.059723 5.901348 2.034980 2.600668 5.413080 4.193725 4.534772 0.377181 4.877840 0.370939 1.298542 3.509873 2.646440 0.087872 0.072299 1.139018 0.864479 0.390688 0.322761 0.625409 0.496780 0.532488 0.232460 0.169219 0.755219 0.379926 0.020447 0.023282 0.503875 0.577513 0.109318 0.153776 0.696533 0.398817 0.008940 0.043707 0.436013 0.087640 0.064863 0.036426 1.673207 0.124068 0.218118 0.039217 0.104335 0.349195 0.838324 0.888693 0.488389 1.385133 0.050226 0.962470 0.502294 1.065585 8.351808 0.377304 5.102837 0.561690 7.010411 3.054968 0.039318 0.204155 2.653232 0.564368 0.854294 15.559906 0.401070 8.929538 5.525874 0.067505 0.273372 0.437116 1.927515 0.940458 2.508169 1.357738 0.043394 0.023126 0.567639 1.048288 0.120994 0.180650 0.449074 3.135353 0.012695 0.570771 2.319555 1.856122 0.975427 3.404087 0.015631 0.458799 0.151684 4.154750 11.429924 1.457957 0.233109 0.077004 0.011074 0.026268 0.052132 8.113282 0.377578 0.429221 0.260296 0.222293 0.273138 2.903836 4.731579 0.564762 0.681215 M 3: 0.658412 0.566269 0.854111 0.884454 1.309554 1.272639 1.874713 0.552007 0.227683 0.581512 0.695190 0.967985 0.344015 0.978992 3.427163 2.333253 0.154701 0.221089 2.088785 0.540749 0.058015 5.851132 2.294145 0.182966 0.684164 3.192521 0.528161 1.128882 3.010922 1.012866 0.227296 0.156635 0.878405 0.802754 0.830884 0.431617 0.456530 3.060574 1.279257 1.438430 0.431464 2.075952 4.840271 0.644656 0.266076 2.084975 0.720060 0.291854 0.028961 4.071574 2.258357 0.073037 1.238426 0.199728 0.160296 0.482619 2.992763 1.296206 0.841829 0.031467 0.048542 0.132774 0.133055 0.056045 0.209188 0.925172 0.360522 0.094591 0.313945 0.118104 0.992259 0.086318 2.149634 5.103188 3.775817 3.954021 0.190734 1.776095 4.495841 0.264277 7.063879 2.221150 3.017954 8.558815 4.310199 2.130054 0.571406 4.137385 0.437589 2.071689 2.498630 1.763546 0.116381 0.296578 1.033710 1.283423 0.312579 0.305772 0.681277 0.507160 0.351381 0.189152 0.217780 0.767361 0.278392 0.092075 0.177263 0.451893 0.653836 0.074620 0.181992 0.752277 0.679853 0.025780 0.082005 0.326441 0.343977 0.195877 0.217424 3.057583 0.377558 0.401252 0.072258 0.241015 0.665865 1.266791 0.680174 0.717301 4.001286 0.362942 1.189259 0.964545 1.350568 12.869737 0.531100 8.904999 0.652629 10.091413 2.671718 0.086367 0.359932 4.797423 0.336801 1.021885 23.029406 0.440178 14.013035 5.069337 0.539010 0.742569 0.780580 1.331875 1.531589 4.414850 1.082703 0.091278 0.172734 0.693405 1.422571 0.068958 0.163829 0.481711 4.643214 0.121821 0.584083 4.216178 1.677263 1.575754 5.046403 0.161015 1.531223 0.599244 5.832025 33.873091 1.914768 1.287474 0.444362 0.076328 0.079916 0.466823 5.231362 0.548763 0.831890 0.382271 0.208791 0.307846 3.717971 5.910440 0.282540 0.964421 Marginal ancestral probabilities saved to: /truba/home/emrah/WORKFOLDER/PROD/300621_run/phylogeny-snakemake/results/Q9BSJ1/4_raxmlng_ancestral/Q9BSJ1.raxml.ancestralProbs Reconstructed ancestral sequences saved to: /truba/home/emrah/WORKFOLDER/PROD/300621_run/phylogeny-snakemake/results/Q9BSJ1/4_raxmlng_ancestral/Q9BSJ1.raxml.ancestralStates Node-labeled tree saved to: /truba/home/emrah/WORKFOLDER/PROD/300621_run/phylogeny-snakemake/results/Q9BSJ1/4_raxmlng_ancestral/Q9BSJ1.raxml.ancestralTree Execution log saved to: /truba/home/emrah/WORKFOLDER/PROD/300621_run/phylogeny-snakemake/results/Q9BSJ1/4_raxmlng_ancestral/Q9BSJ1.raxml.log Analysis started: 12-Jul-2021 17:29:01 / finished: 12-Jul-2021 17:29:53 Elapsed time: 51.203 seconds Consumed energy: 3.411 Wh