RAxML-NG v. 1.0.2 released on 22.02.2021 by The Exelixis Lab. Developed by: Alexey M. Kozlov and Alexandros Stamatakis. Contributors: Diego Darriba, Tomas Flouri, Benoit Morel, Sarah Lutteropp, Ben Bettisworth. Latest version: https://github.com/amkozlov/raxml-ng Questions/problems/suggestions? Please visit: https://groups.google.com/forum/#!forum/raxml System: Intel(R) Xeon(R) Gold 6148 CPU @ 2.40GHz, 40 cores, 376 GB RAM RAxML-NG was called at 03-Jun-2021 14:59:53 as follows: raxml-ng --ancestral --msa /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/Q96PY6/2_msa/Q96PY6_nogap_msa.fasta --tree /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/Q96PY6/3_mltree/Q96PY6.raxml.bestTree_unrooted --model LG4X --prefix /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/Q96PY6/4_raxmlng_ancestral/Q96PY6 --threads 4 Analysis options: run mode: Ancestral state reconstruction start tree(s): user random seed: 1622721593 tip-inner: ON pattern compression: OFF per-rate scalers: OFF site repeats: OFF branch lengths: proportional (ML estimate, algorithm: NR-FAST) SIMD kernels: AVX2 parallelization: coarse-grained (auto), PTHREADS (4 threads), thread pinning: OFF [00:00:00] Reading alignment from file: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/Q96PY6/2_msa/Q96PY6_nogap_msa.fasta [00:00:00] Loaded alignment with 1000 taxa and 1258 sites WARNING: Sequences tr_G3S0W8_G3S0W8_GORGO_9595 and tr_H2NLU1_H2NLU1_PONAB_9601 are exactly identical! WARNING: Sequences tr_G3S0W8_G3S0W8_GORGO_9595 and tr_K7CFQ0_K7CFQ0_PANTR_9598 are exactly identical! WARNING: Sequences tr_G3S0W8_G3S0W8_GORGO_9595 and sp_Q8TD19_NEK9_HUMAN_9606 are exactly identical! WARNING: Sequences tr_G3S0W8_G3S0W8_GORGO_9595 and tr_A0A2R8ZH82_A0A2R8ZH82_PANPA_9597 are exactly identical! WARNING: Sequences tr_A0A2J8L8I7_A0A2J8L8I7_PANTR_9598 and sp_P51956_NEK3_HUMAN_9606 are exactly identical! WARNING: Sequences tr_A0A2J8L8I7_A0A2J8L8I7_PANTR_9598 and tr_A0A2R9BI97_A0A2R9BI97_PANPA_9597 are exactly identical! WARNING: Sequences tr_A0A0E0HXB1_A0A0E0HXB1_ORYNI_4536 and tr_B8B7P0_B8B7P0_ORYSI_39946 are exactly identical! WARNING: Sequences tr_A0A0E0HXB1_A0A0E0HXB1_ORYNI_4536 and tr_A0A0E0Q4R1_A0A0E0Q4R1_ORYRU_4529 are exactly identical! WARNING: Sequences tr_A0A0E0HXB1_A0A0E0HXB1_ORYNI_4536 and tr_A0A0E0AGF0_A0A0E0AGF0_9ORYZ_40148 are exactly identical! WARNING: Sequences tr_A0A0E0HXB1_A0A0E0HXB1_ORYNI_4536 and sp_Q6ZEZ5_NEK3_ORYSJ_39947 are exactly identical! WARNING: Sequences tr_A0A0E0JC51_A0A0E0JC51_ORYNI_4536 and tr_A0A0E0RJQ5_A0A0E0RJQ5_ORYRU_4529 are exactly identical! WARNING: Sequences tr_A0A0E0JC51_A0A0E0JC51_ORYNI_4536 and sp_Q2QMH1_NEK2_ORYSJ_39947 are exactly identical! WARNING: Sequences tr_I1R7P0_I1R7P0_ORYGL_4538 and tr_A0A0E0FDY0_A0A0E0FDY0_9ORYZ_40149 are exactly identical! WARNING: Sequences tr_I1R7P0_I1R7P0_ORYGL_4538 and tr_A0A0D3HWJ0_A0A0D3HWJ0_9ORYZ_65489 are exactly identical! WARNING: Sequences tr_A0A0E0NYL6_A0A0E0NYL6_ORYRU_4529 and sp_Q10GB1_NEK1_ORYSJ_39947 are exactly identical! WARNING: Sequences tr_B3RKK0_B3RKK0_TRIAD_10228 and tr_A0A369SCZ5_A0A369SCZ5_9METZ_287889 are exactly identical! WARNING: Sequences tr_G7PTY4_G7PTY4_MACFA_9541 and tr_A0A2K5NPU5_A0A2K5NPU5_CERAT_9531 are exactly identical! WARNING: Sequences tr_G7PTY4_G7PTY4_MACFA_9541 and tr_A0A2K6DDI2_A0A2K6DDI2_MACNE_9545 are exactly identical! WARNING: Sequences tr_G7PTY4_G7PTY4_MACFA_9541 and tr_A0A2K5XGK4_A0A2K5XGK4_MANLE_9568 are exactly identical! WARNING: Sequences tr_A0A1S3Z119_A0A1S3Z119_TOBAC_4097 and tr_A0A1U7VXL2_A0A1U7VXL2_NICSY_4096 are exactly identical! WARNING: Sequences tr_A0A1S4DIJ5_A0A1S4DIJ5_TOBAC_4097 and tr_A0A1U7XGW0_A0A1U7XGW0_NICSY_4096 are exactly identical! WARNING: Sequences tr_A0A1S3VF22_A0A1S3VF22_VIGRR_3916 and tr_A0A3Q0FJR5_A0A3Q0FJR5_VIGRR_3916 are exactly identical! WARNING: Sequences tr_A0A2D0R5B4_A0A2D0R5B4_ICTPU_7998 and tr_A0A2D0R5E3_A0A2D0R5E3_ICTPU_7998 are exactly identical! WARNING: Duplicate sequences found: 23 NOTE: Reduced alignment (with duplicates and gap-only sites/taxa removed) NOTE: was saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/Q96PY6/4_raxmlng_ancestral/Q96PY6.raxml.reduced.phy Alignment comprises 1 partitions and 1258 sites Partition 0: noname Model: LG4X+R4 Alignment sites: 1258 Gaps: 54.94 % Invariant sites: 0.08 % NOTE: Binary MSA file created: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/Q96PY6/4_raxmlng_ancestral/Q96PY6.raxml.rba Parallelization scheme autoconfig: 1 worker(s) x 4 thread(s) Parallel reduction/worker buffer size: 1 KB / 0 KB [00:00:00] Loading user starting tree(s) from: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/Q96PY6/3_mltree/Q96PY6.raxml.bestTree_unrooted [00:00:00] Data distribution: max. partitions/sites/weight per thread: 1 / 315 / 25200 [00:00:00] Data distribution: max. searches per worker: 1 Starting ML tree search with 1 distinct starting trees [00:00:00] Tree #1, initial LogLikelihood: -474037.837478 [00:00:00 -474037.837478] Initial branch length optimization [00:00:03 -455686.253593] Model parameter optimization (eps = 0.100000) [00:01:14] Tree #1, final logLikelihood: -454254.750155 Optimized model parameters: Partition 0: noname Rate heterogeneity: FREE (4 cats, mean), weights&rates: (0.066764,0.176083) (0.068503,0.185694) (0.197088,0.616378) (0.667644,1.279188) Base frequencies (model): M0: 0.147383 0.017579 0.058208 0.017707 0.026331 0.041582 0.017494 0.027859 0.011849 0.076971 0.147823 0.019535 0.037132 0.029940 0.008059 0.088179 0.089653 0.006477 0.032308 0.097931 M1: 0.063139 0.066357 0.011586 0.066571 0.010800 0.009276 0.053984 0.146986 0.034214 0.088822 0.098196 0.032390 0.021263 0.072697 0.016761 0.020711 0.020797 0.025463 0.045615 0.094372 M2: 0.062457 0.066826 0.049332 0.065270 0.006513 0.041231 0.058965 0.080852 0.028024 0.037024 0.075925 0.064131 0.019620 0.028710 0.104579 0.056388 0.062027 0.008241 0.033124 0.050760 M3: 0.106471 0.074171 0.044513 0.096390 0.002148 0.066733 0.158908 0.037625 0.020691 0.014608 0.028797 0.105352 0.007864 0.007477 0.083595 0.055726 0.047711 0.003975 0.010088 0.027159 Substitution rates (model): M 0: 0.295719 0.067388 0.253712 1.029289 0.107964 0.514644 10.868848 0.380498 0.084223 0.086976 0.188789 0.286389 0.155567 1.671061 2.132922 0.529591 0.115551 0.102453 0.916683 0.448317 0.457483 0.576016 1.741924 0.736017 0.704334 5.658311 0.123387 0.221777 93.433377 0.382175 0.235965 6.535048 0.525521 0.303537 0.641259 0.289466 0.102065 2.358429 0.251987 0.216561 0.503084 0.435271 4.873453 0.090748 0.033310 0.746537 0.128905 0.127321 0.904011 0.939733 0.435450 0.046646 0.262076 0.043986 0.189008 0.599450 109.901504 1.070052 5.229858 0.052764 0.021407 0.621146 0.081091 0.205164 5.164456 0.747330 0.308078 0.260889 0.185083 0.080708 0.029955 0.084794 1.862626 0.553477 0.151733 0.230320 0.096955 0.352526 0.590018 0.386853 1.559564 0.606648 0.587531 0.592318 0.885230 4.117654 0.246260 6.508329 0.054187 0.195703 1.669092 0.810168 0.066081 2.437439 0.165666 0.106333 0.093417 0.035149 0.072549 1.202023 1.634845 0.060194 0.069359 2.448827 0.232297 0.064822 3.537387 0.435384 0.290413 0.280695 0.105999 0.206603 0.404968 0.048984 0.069963 0.256662 0.228519 0.241077 4.320442 3.656545 0.290216 0.307466 0.096556 0.306067 0.204296 0.504221 1.991533 0.655465 6.799829 11.291065 0.961142 0.448965 6.227274 20.304886 0.205944 1.495537 0.091940 1.994320 0.754940 0.170343 0.050315 0.372166 0.206332 0.097050 5.381403 0.122332 3.256485 2.261319 0.848067 0.064441 0.102493 0.459041 0.133091 0.561215 0.457430 0.163849 5.260446 0.360946 0.389413 0.033291 0.115301 0.112593 1.559944 0.426508 0.132547 0.498634 0.559069 0.264728 0.693307 0.438856 0.306683 0.109129 18.392863 66.647302 0.400021 4.586081 2.099355 0.411347 0.476350 0.584622 3.634276 0.101797 0.148995 0.089177 0.034710 0.063603 0.755865 20.561407 0.133790 0.154902 M 1: 0.066142 0.590377 0.069930 9.850951 1.101363 0.150375 0.568586 0.051668 0.127170 0.292429 0.071458 1.218562 0.075144 7.169085 30.139501 13.461692 0.021372 0.045779 4.270235 0.468325 0.013688 0.302287 1.353957 0.028386 0.037750 0.262130 0.016923 0.064289 0.855973 0.079621 0.011169 0.161937 0.276530 0.161053 0.081472 0.036742 0.030342 2.851667 3.932151 8.159169 0.219934 0.421974 2.468752 0.344765 0.210724 1.172204 0.763553 0.082464 0.726566 11.149790 4.782635 0.058046 0.498072 0.258487 0.146882 0.249672 0.560142 0.046719 0.106259 0.003656 0.004200 0.014189 0.009876 0.002656 0.040244 0.267322 0.053740 0.006597 0.027639 0.012745 0.582670 0.005035 0.275844 0.098208 0.445038 1.217010 0.033969 1.988516 0.681161 0.825960 18.762977 11.949233 0.286794 0.534219 4.336817 3.054085 0.129551 4.210126 0.165753 1.088704 1.889645 3.344809 0.111063 2.067758 3.547017 2.466507 0.188236 0.203493 0.281953 0.037250 0.029788 0.008541 0.014768 0.125869 0.056702 0.004186 0.110993 0.201148 0.139705 0.009201 0.012095 0.043812 0.013513 0.002533 0.005848 0.031390 0.021612 0.004854 0.129497 0.976631 0.053397 0.019475 0.004964 0.015539 0.031779 0.064558 0.065585 0.079927 0.095591 0.196886 0.408834 0.126088 0.037226 0.452302 0.016212 7.278994 0.029917 7.918203 0.450964 0.169797 0.104288 1.578530 0.015909 0.094365 16.179952 0.042762 14.799537 1.506485 0.637893 0.123793 0.641351 0.154810 0.140750 3.416059 0.259400 0.009457 0.090576 0.292108 0.297913 0.017172 0.021976 0.032578 1.375871 0.457399 0.598048 4.418398 0.239749 0.168432 2.950318 0.143327 0.328689 0.125011 0.562720 1.414883 0.227807 3.478333 2.984862 0.061299 0.077470 1.050562 13.974326 0.154326 0.224675 0.112000 0.060703 0.123480 5.294490 0.447011 0.033381 0.045528 M 2: 0.733336 0.558955 0.503360 4.149599 1.415369 1.367574 1.263002 0.994098 0.517204 0.775054 0.763094 1.890137 0.540460 0.200122 4.972745 1.825593 0.450842 0.526135 3.839269 0.597671 0.058964 2.863355 2.872594 0.258365 0.366868 2.578946 0.358350 0.672023 5.349861 0.691594 0.063347 0.032875 0.821562 0.580847 0.661866 0.265730 0.395134 5.581680 1.279881 1.335650 0.397108 1.840061 5.739035 0.284730 0.109781 1.612642 0.466979 0.141582 0.019509 4.670980 1.967383 0.088064 0.581928 0.145401 0.225860 0.434096 2.292917 1.024707 0.821921 0.027824 0.021443 0.088850 0.060820 0.018288 0.042687 1.199607 0.420710 0.037642 0.141233 0.090101 1.043232 0.209978 0.823594 3.039380 1.463390 1.983693 0.397640 2.831098 4.102068 0.059723 5.901348 2.034980 2.600668 5.413080 4.193725 4.534772 0.377181 4.877840 0.370939 1.298542 3.509873 2.646440 0.087872 0.072299 1.139018 0.864479 0.390688 0.322761 0.625409 0.496780 0.532488 0.232460 0.169219 0.755219 0.379926 0.020447 0.023282 0.503875 0.577513 0.109318 0.153776 0.696533 0.398817 0.008940 0.043707 0.436013 0.087640 0.064863 0.036426 1.673207 0.124068 0.218118 0.039217 0.104335 0.349195 0.838324 0.888693 0.488389 1.385133 0.050226 0.962470 0.502294 1.065585 8.351808 0.377304 5.102837 0.561690 7.010411 3.054968 0.039318 0.204155 2.653232 0.564368 0.854294 15.559906 0.401070 8.929538 5.525874 0.067505 0.273372 0.437116 1.927515 0.940458 2.508169 1.357738 0.043394 0.023126 0.567639 1.048288 0.120994 0.180650 0.449074 3.135353 0.012695 0.570771 2.319555 1.856122 0.975427 3.404087 0.015631 0.458799 0.151684 4.154750 11.429924 1.457957 0.233109 0.077004 0.011074 0.026268 0.052132 8.113282 0.377578 0.429221 0.260296 0.222293 0.273138 2.903836 4.731579 0.564762 0.681215 M 3: 0.658412 0.566269 0.854111 0.884454 1.309554 1.272639 1.874713 0.552007 0.227683 0.581512 0.695190 0.967985 0.344015 0.978992 3.427163 2.333253 0.154701 0.221089 2.088785 0.540749 0.058015 5.851132 2.294145 0.182966 0.684164 3.192521 0.528161 1.128882 3.010922 1.012866 0.227296 0.156635 0.878405 0.802754 0.830884 0.431617 0.456530 3.060574 1.279257 1.438430 0.431464 2.075952 4.840271 0.644656 0.266076 2.084975 0.720060 0.291854 0.028961 4.071574 2.258357 0.073037 1.238426 0.199728 0.160296 0.482619 2.992763 1.296206 0.841829 0.031467 0.048542 0.132774 0.133055 0.056045 0.209188 0.925172 0.360522 0.094591 0.313945 0.118104 0.992259 0.086318 2.149634 5.103188 3.775817 3.954021 0.190734 1.776095 4.495841 0.264277 7.063879 2.221150 3.017954 8.558815 4.310199 2.130054 0.571406 4.137385 0.437589 2.071689 2.498630 1.763546 0.116381 0.296578 1.033710 1.283423 0.312579 0.305772 0.681277 0.507160 0.351381 0.189152 0.217780 0.767361 0.278392 0.092075 0.177263 0.451893 0.653836 0.074620 0.181992 0.752277 0.679853 0.025780 0.082005 0.326441 0.343977 0.195877 0.217424 3.057583 0.377558 0.401252 0.072258 0.241015 0.665865 1.266791 0.680174 0.717301 4.001286 0.362942 1.189259 0.964545 1.350568 12.869737 0.531100 8.904999 0.652629 10.091413 2.671718 0.086367 0.359932 4.797423 0.336801 1.021885 23.029406 0.440178 14.013035 5.069337 0.539010 0.742569 0.780580 1.331875 1.531589 4.414850 1.082703 0.091278 0.172734 0.693405 1.422571 0.068958 0.163829 0.481711 4.643214 0.121821 0.584083 4.216178 1.677263 1.575754 5.046403 0.161015 1.531223 0.599244 5.832025 33.873091 1.914768 1.287474 0.444362 0.076328 0.079916 0.466823 5.231362 0.548763 0.831890 0.382271 0.208791 0.307846 3.717971 5.910440 0.282540 0.964421 Marginal ancestral probabilities saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/Q96PY6/4_raxmlng_ancestral/Q96PY6.raxml.ancestralProbs Reconstructed ancestral sequences saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/Q96PY6/4_raxmlng_ancestral/Q96PY6.raxml.ancestralStates Node-labeled tree saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/Q96PY6/4_raxmlng_ancestral/Q96PY6.raxml.ancestralTree Execution log saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/Q96PY6/4_raxmlng_ancestral/Q96PY6.raxml.log Analysis started: 03-Jun-2021 14:59:53 / finished: 03-Jun-2021 15:01:20 Elapsed time: 87.590 seconds Consumed energy: 7.471 Wh