RAxML-NG v. 1.0.2 released on 22.02.2021 by The Exelixis Lab. Developed by: Alexey M. Kozlov and Alexandros Stamatakis. Contributors: Diego Darriba, Tomas Flouri, Benoit Morel, Sarah Lutteropp, Ben Bettisworth. Latest version: https://github.com/amkozlov/raxml-ng Questions/problems/suggestions? Please visit: https://groups.google.com/forum/#!forum/raxml System: Intel(R) Xeon(R) Gold 6148 CPU @ 2.40GHz, 40 cores, 376 GB RAM RAxML-NG was called at 03-Jun-2021 02:53:27 as follows: raxml-ng --ancestral --msa /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P42224/2_msa/P42224_nogap_msa.fasta --tree /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P42224/3_mltree/P42224.raxml.bestTree_unrooted --model LG4X --prefix /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P42224/4_raxmlng_ancestral/P42224 --threads 4 Analysis options: run mode: Ancestral state reconstruction start tree(s): user random seed: 1622678007 tip-inner: ON pattern compression: OFF per-rate scalers: OFF site repeats: OFF branch lengths: proportional (ML estimate, algorithm: NR-FAST) SIMD kernels: AVX2 parallelization: coarse-grained (auto), PTHREADS (4 threads), thread pinning: OFF [00:00:00] Reading alignment from file: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P42224/2_msa/P42224_nogap_msa.fasta [00:00:00] Loaded alignment with 538 taxa and 750 sites WARNING: Sequences sp_Q6DV79_STAT3_CHICK_9031 and tr_G1MWW8_G1MWW8_MELGA_9103 are exactly identical! WARNING: Sequences sp_Q6DV79_STAT3_CHICK_9031 and tr_A0A226MJN4_A0A226MJN4_CALSU_9009 are exactly identical! WARNING: Sequences sp_Q6DV79_STAT3_CHICK_9031 and tr_A0A226NTZ7_A0A226NTZ7_COLVI_9014 are exactly identical! WARNING: Sequences sp_P42227_STAT3_MOUSE_10090 and tr_G1RLB0_G1RLB0_NOMLE_61853 are exactly identical! WARNING: Sequences sp_P42227_STAT3_MOUSE_10090 and tr_G3HLW9_G3HLW9_CRIGR_10029 are exactly identical! WARNING: Sequences sp_P42227_STAT3_MOUSE_10090 and tr_G1T0H3_G1T0H3_RABIT_9986 are exactly identical! WARNING: Sequences sp_P42227_STAT3_MOUSE_10090 and sp_P40763_STAT3_HUMAN_9606 are exactly identical! WARNING: Sequences sp_P42227_STAT3_MOUSE_10090 and tr_U3BQV2_U3BQV2_CALJA_9483 are exactly identical! WARNING: Sequences sp_P42227_STAT3_MOUSE_10090 and tr_M3VY01_M3VY01_FELCA_9685 are exactly identical! WARNING: Sequences sp_P42227_STAT3_MOUSE_10090 and tr_A0A2I3MP47_A0A2I3MP47_PAPAN_9555 are exactly identical! WARNING: Sequences sp_P42227_STAT3_MOUSE_10090 and tr_A0A0D9S1T9_A0A0D9S1T9_CHLSB_60711 are exactly identical! WARNING: Sequences sp_P42227_STAT3_MOUSE_10090 and tr_A0A2U4BPF6_A0A2U4BPF6_TURTR_9739 are exactly identical! WARNING: Sequences sp_P42227_STAT3_MOUSE_10090 and tr_A0A2Y9EI55_A0A2Y9EI55_PHYCD_9755 are exactly identical! WARNING: Sequences sp_P42227_STAT3_MOUSE_10090 and tr_A0A384CYE5_A0A384CYE5_URSMA_29073 are exactly identical! WARNING: Sequences tr_M3YGH5_M3YGH5_MUSPF_9669 and tr_A0A2Y9K559_A0A2Y9K559_ENHLU_391180 are exactly identical! WARNING: Sequences tr_H2P855_H2P855_PONAB_9601 and sp_P42224_STAT1_HUMAN_9606 are exactly identical! WARNING: Sequences tr_K7B230_K7B230_PANTR_9598 and sp_Q14765_STAT4_HUMAN_9606 are exactly identical! WARNING: Sequences tr_A0A1D5QPR1_A0A1D5QPR1_MACMU_9544 and tr_A0A2I3LSU8_A0A2I3LSU8_PAPAN_9555 are exactly identical! WARNING: Sequences tr_A0A1D5R9X3_A0A1D5R9X3_MACMU_9544 and tr_A0A2I3LSC8_A0A2I3LSC8_PAPAN_9555 are exactly identical! WARNING: Sequences tr_A0A1D5R9X3_A0A1D5R9X3_MACMU_9544 and tr_A0A2K6APQ6_A0A2K6APQ6_MACNE_9545 are exactly identical! WARNING: Sequences tr_F6R1P6_F6R1P6_MACMU_9544 and tr_G7PIH9_G7PIH9_MACFA_9541 are exactly identical! WARNING: Sequences tr_A0A096N8T7_A0A096N8T7_PAPAN_9555 and tr_A0A2K5YN84_A0A2K5YN84_MANLE_9568 are exactly identical! WARNING: Sequences tr_A0A151NB22_A0A151NB22_ALLMI_8496 and tr_A0A1U8DHZ7_A0A1U8DHZ7_ALLSI_38654 are exactly identical! WARNING: Sequences tr_A0A151PC08_A0A151PC08_ALLMI_8496 and tr_A0A3Q0GY41_A0A3Q0GY41_ALLSI_38654 are exactly identical! WARNING: Sequences tr_A0A091FR98_A0A091FR98_9AVES_55661 and tr_A0A2I0LK33_A0A2I0LK33_COLLI_8932 are exactly identical! WARNING: Sequences tr_A0A091FR98_A0A091FR98_9AVES_55661 and tr_A0A091HNR2_A0A091HNR2_CALAN_9244 are exactly identical! WARNING: Sequences tr_A0A091FR98_A0A091FR98_9AVES_55661 and tr_A0A1V4JQS7_A0A1V4JQS7_PATFA_372326 are exactly identical! WARNING: Sequences tr_A0A091FR98_A0A091FR98_9AVES_55661 and tr_A0A218UMN7_A0A218UMN7_9PASE_299123 are exactly identical! WARNING: Sequences tr_A0A2D0Q888_A0A2D0Q888_ICTPU_7998 and tr_A0A2D0QAV2_A0A2D0QAV2_ICTPU_7998 are exactly identical! WARNING: Sequences tr_A0A2D0R5V2_A0A2D0R5V2_ICTPU_7998 and tr_A0A2D0R828_A0A2D0R828_ICTPU_7998 are exactly identical! WARNING: Duplicate sequences found: 30 NOTE: Reduced alignment (with duplicates and gap-only sites/taxa removed) NOTE: was saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P42224/4_raxmlng_ancestral/P42224.raxml.reduced.phy Alignment comprises 1 partitions and 750 sites Partition 0: noname Model: LG4X+R4 Alignment sites: 750 Gaps: 16.40 % Invariant sites: 0.13 % NOTE: Binary MSA file created: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P42224/4_raxmlng_ancestral/P42224.raxml.rba Parallelization scheme autoconfig: 1 worker(s) x 4 thread(s) Parallel reduction/worker buffer size: 1 KB / 0 KB [00:00:00] Loading user starting tree(s) from: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P42224/3_mltree/P42224.raxml.bestTree_unrooted [00:00:00] Data distribution: max. partitions/sites/weight per thread: 1 / 188 / 15040 [00:00:00] Data distribution: max. searches per worker: 1 Starting ML tree search with 1 distinct starting trees [00:00:00] Tree #1, initial LogLikelihood: -115738.595045 [00:00:00 -115738.595045] Initial branch length optimization [00:00:00 -115480.951860] Model parameter optimization (eps = 0.100000) [00:00:26] Tree #1, final logLikelihood: -115077.305491 Optimized model parameters: Partition 0: noname Rate heterogeneity: FREE (4 cats, mean), weights&rates: (0.155004,0.430155) (0.134750,0.457508) (0.416526,0.768564) (0.293721,1.877800) Base frequencies (model): M0: 0.147383 0.017579 0.058208 0.017707 0.026331 0.041582 0.017494 0.027859 0.011849 0.076971 0.147823 0.019535 0.037132 0.029940 0.008059 0.088179 0.089653 0.006477 0.032308 0.097931 M1: 0.063139 0.066357 0.011586 0.066571 0.010800 0.009276 0.053984 0.146986 0.034214 0.088822 0.098196 0.032390 0.021263 0.072697 0.016761 0.020711 0.020797 0.025463 0.045615 0.094372 M2: 0.062457 0.066826 0.049332 0.065270 0.006513 0.041231 0.058965 0.080852 0.028024 0.037024 0.075925 0.064131 0.019620 0.028710 0.104579 0.056388 0.062027 0.008241 0.033124 0.050760 M3: 0.106471 0.074171 0.044513 0.096390 0.002148 0.066733 0.158908 0.037625 0.020691 0.014608 0.028797 0.105352 0.007864 0.007477 0.083595 0.055726 0.047711 0.003975 0.010088 0.027159 Substitution rates (model): M 0: 0.295719 0.067388 0.253712 1.029289 0.107964 0.514644 10.868848 0.380498 0.084223 0.086976 0.188789 0.286389 0.155567 1.671061 2.132922 0.529591 0.115551 0.102453 0.916683 0.448317 0.457483 0.576016 1.741924 0.736017 0.704334 5.658311 0.123387 0.221777 93.433377 0.382175 0.235965 6.535048 0.525521 0.303537 0.641259 0.289466 0.102065 2.358429 0.251987 0.216561 0.503084 0.435271 4.873453 0.090748 0.033310 0.746537 0.128905 0.127321 0.904011 0.939733 0.435450 0.046646 0.262076 0.043986 0.189008 0.599450 109.901504 1.070052 5.229858 0.052764 0.021407 0.621146 0.081091 0.205164 5.164456 0.747330 0.308078 0.260889 0.185083 0.080708 0.029955 0.084794 1.862626 0.553477 0.151733 0.230320 0.096955 0.352526 0.590018 0.386853 1.559564 0.606648 0.587531 0.592318 0.885230 4.117654 0.246260 6.508329 0.054187 0.195703 1.669092 0.810168 0.066081 2.437439 0.165666 0.106333 0.093417 0.035149 0.072549 1.202023 1.634845 0.060194 0.069359 2.448827 0.232297 0.064822 3.537387 0.435384 0.290413 0.280695 0.105999 0.206603 0.404968 0.048984 0.069963 0.256662 0.228519 0.241077 4.320442 3.656545 0.290216 0.307466 0.096556 0.306067 0.204296 0.504221 1.991533 0.655465 6.799829 11.291065 0.961142 0.448965 6.227274 20.304886 0.205944 1.495537 0.091940 1.994320 0.754940 0.170343 0.050315 0.372166 0.206332 0.097050 5.381403 0.122332 3.256485 2.261319 0.848067 0.064441 0.102493 0.459041 0.133091 0.561215 0.457430 0.163849 5.260446 0.360946 0.389413 0.033291 0.115301 0.112593 1.559944 0.426508 0.132547 0.498634 0.559069 0.264728 0.693307 0.438856 0.306683 0.109129 18.392863 66.647302 0.400021 4.586081 2.099355 0.411347 0.476350 0.584622 3.634276 0.101797 0.148995 0.089177 0.034710 0.063603 0.755865 20.561407 0.133790 0.154902 M 1: 0.066142 0.590377 0.069930 9.850951 1.101363 0.150375 0.568586 0.051668 0.127170 0.292429 0.071458 1.218562 0.075144 7.169085 30.139501 13.461692 0.021372 0.045779 4.270235 0.468325 0.013688 0.302287 1.353957 0.028386 0.037750 0.262130 0.016923 0.064289 0.855973 0.079621 0.011169 0.161937 0.276530 0.161053 0.081472 0.036742 0.030342 2.851667 3.932151 8.159169 0.219934 0.421974 2.468752 0.344765 0.210724 1.172204 0.763553 0.082464 0.726566 11.149790 4.782635 0.058046 0.498072 0.258487 0.146882 0.249672 0.560142 0.046719 0.106259 0.003656 0.004200 0.014189 0.009876 0.002656 0.040244 0.267322 0.053740 0.006597 0.027639 0.012745 0.582670 0.005035 0.275844 0.098208 0.445038 1.217010 0.033969 1.988516 0.681161 0.825960 18.762977 11.949233 0.286794 0.534219 4.336817 3.054085 0.129551 4.210126 0.165753 1.088704 1.889645 3.344809 0.111063 2.067758 3.547017 2.466507 0.188236 0.203493 0.281953 0.037250 0.029788 0.008541 0.014768 0.125869 0.056702 0.004186 0.110993 0.201148 0.139705 0.009201 0.012095 0.043812 0.013513 0.002533 0.005848 0.031390 0.021612 0.004854 0.129497 0.976631 0.053397 0.019475 0.004964 0.015539 0.031779 0.064558 0.065585 0.079927 0.095591 0.196886 0.408834 0.126088 0.037226 0.452302 0.016212 7.278994 0.029917 7.918203 0.450964 0.169797 0.104288 1.578530 0.015909 0.094365 16.179952 0.042762 14.799537 1.506485 0.637893 0.123793 0.641351 0.154810 0.140750 3.416059 0.259400 0.009457 0.090576 0.292108 0.297913 0.017172 0.021976 0.032578 1.375871 0.457399 0.598048 4.418398 0.239749 0.168432 2.950318 0.143327 0.328689 0.125011 0.562720 1.414883 0.227807 3.478333 2.984862 0.061299 0.077470 1.050562 13.974326 0.154326 0.224675 0.112000 0.060703 0.123480 5.294490 0.447011 0.033381 0.045528 M 2: 0.733336 0.558955 0.503360 4.149599 1.415369 1.367574 1.263002 0.994098 0.517204 0.775054 0.763094 1.890137 0.540460 0.200122 4.972745 1.825593 0.450842 0.526135 3.839269 0.597671 0.058964 2.863355 2.872594 0.258365 0.366868 2.578946 0.358350 0.672023 5.349861 0.691594 0.063347 0.032875 0.821562 0.580847 0.661866 0.265730 0.395134 5.581680 1.279881 1.335650 0.397108 1.840061 5.739035 0.284730 0.109781 1.612642 0.466979 0.141582 0.019509 4.670980 1.967383 0.088064 0.581928 0.145401 0.225860 0.434096 2.292917 1.024707 0.821921 0.027824 0.021443 0.088850 0.060820 0.018288 0.042687 1.199607 0.420710 0.037642 0.141233 0.090101 1.043232 0.209978 0.823594 3.039380 1.463390 1.983693 0.397640 2.831098 4.102068 0.059723 5.901348 2.034980 2.600668 5.413080 4.193725 4.534772 0.377181 4.877840 0.370939 1.298542 3.509873 2.646440 0.087872 0.072299 1.139018 0.864479 0.390688 0.322761 0.625409 0.496780 0.532488 0.232460 0.169219 0.755219 0.379926 0.020447 0.023282 0.503875 0.577513 0.109318 0.153776 0.696533 0.398817 0.008940 0.043707 0.436013 0.087640 0.064863 0.036426 1.673207 0.124068 0.218118 0.039217 0.104335 0.349195 0.838324 0.888693 0.488389 1.385133 0.050226 0.962470 0.502294 1.065585 8.351808 0.377304 5.102837 0.561690 7.010411 3.054968 0.039318 0.204155 2.653232 0.564368 0.854294 15.559906 0.401070 8.929538 5.525874 0.067505 0.273372 0.437116 1.927515 0.940458 2.508169 1.357738 0.043394 0.023126 0.567639 1.048288 0.120994 0.180650 0.449074 3.135353 0.012695 0.570771 2.319555 1.856122 0.975427 3.404087 0.015631 0.458799 0.151684 4.154750 11.429924 1.457957 0.233109 0.077004 0.011074 0.026268 0.052132 8.113282 0.377578 0.429221 0.260296 0.222293 0.273138 2.903836 4.731579 0.564762 0.681215 M 3: 0.658412 0.566269 0.854111 0.884454 1.309554 1.272639 1.874713 0.552007 0.227683 0.581512 0.695190 0.967985 0.344015 0.978992 3.427163 2.333253 0.154701 0.221089 2.088785 0.540749 0.058015 5.851132 2.294145 0.182966 0.684164 3.192521 0.528161 1.128882 3.010922 1.012866 0.227296 0.156635 0.878405 0.802754 0.830884 0.431617 0.456530 3.060574 1.279257 1.438430 0.431464 2.075952 4.840271 0.644656 0.266076 2.084975 0.720060 0.291854 0.028961 4.071574 2.258357 0.073037 1.238426 0.199728 0.160296 0.482619 2.992763 1.296206 0.841829 0.031467 0.048542 0.132774 0.133055 0.056045 0.209188 0.925172 0.360522 0.094591 0.313945 0.118104 0.992259 0.086318 2.149634 5.103188 3.775817 3.954021 0.190734 1.776095 4.495841 0.264277 7.063879 2.221150 3.017954 8.558815 4.310199 2.130054 0.571406 4.137385 0.437589 2.071689 2.498630 1.763546 0.116381 0.296578 1.033710 1.283423 0.312579 0.305772 0.681277 0.507160 0.351381 0.189152 0.217780 0.767361 0.278392 0.092075 0.177263 0.451893 0.653836 0.074620 0.181992 0.752277 0.679853 0.025780 0.082005 0.326441 0.343977 0.195877 0.217424 3.057583 0.377558 0.401252 0.072258 0.241015 0.665865 1.266791 0.680174 0.717301 4.001286 0.362942 1.189259 0.964545 1.350568 12.869737 0.531100 8.904999 0.652629 10.091413 2.671718 0.086367 0.359932 4.797423 0.336801 1.021885 23.029406 0.440178 14.013035 5.069337 0.539010 0.742569 0.780580 1.331875 1.531589 4.414850 1.082703 0.091278 0.172734 0.693405 1.422571 0.068958 0.163829 0.481711 4.643214 0.121821 0.584083 4.216178 1.677263 1.575754 5.046403 0.161015 1.531223 0.599244 5.832025 33.873091 1.914768 1.287474 0.444362 0.076328 0.079916 0.466823 5.231362 0.548763 0.831890 0.382271 0.208791 0.307846 3.717971 5.910440 0.282540 0.964421 Marginal ancestral probabilities saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P42224/4_raxmlng_ancestral/P42224.raxml.ancestralProbs Reconstructed ancestral sequences saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P42224/4_raxmlng_ancestral/P42224.raxml.ancestralStates Node-labeled tree saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P42224/4_raxmlng_ancestral/P42224.raxml.ancestralTree Execution log saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P42224/4_raxmlng_ancestral/P42224.raxml.log Analysis started: 03-Jun-2021 02:53:27 / finished: 03-Jun-2021 02:53:57 Elapsed time: 29.869 seconds Consumed energy: 2.376 Wh