RAxML-NG v. 1.0.2 released on 22.02.2021 by The Exelixis Lab. Developed by: Alexey M. Kozlov and Alexandros Stamatakis. Contributors: Diego Darriba, Tomas Flouri, Benoit Morel, Sarah Lutteropp, Ben Bettisworth. Latest version: https://github.com/amkozlov/raxml-ng Questions/problems/suggestions? Please visit: https://groups.google.com/forum/#!forum/raxml System: Intel(R) Xeon(R) Gold 6148 CPU @ 2.40GHz, 40 cores, 376 GB RAM RAxML-NG was called at 03-Jun-2021 02:07:24 as follows: raxml-ng --ancestral --msa /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P00480/2_msa/P00480_nogap_msa.fasta --tree /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P00480/3_mltree/P00480.raxml.bestTree_unrooted --model LG4X --prefix /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P00480/4_raxmlng_ancestral/P00480 --threads 4 Analysis options: run mode: Ancestral state reconstruction start tree(s): user random seed: 1622675244 tip-inner: ON pattern compression: OFF per-rate scalers: OFF site repeats: OFF branch lengths: proportional (ML estimate, algorithm: NR-FAST) SIMD kernels: AVX2 parallelization: coarse-grained (auto), PTHREADS (4 threads), thread pinning: OFF [00:00:00] Reading alignment from file: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P00480/2_msa/P00480_nogap_msa.fasta [00:00:00] Loaded alignment with 848 taxa and 354 sites WARNING: Sequences sp_P0CL21_OTC_COCIM_246410 and tr_A0A0J6YMG9_A0A0J6YMG9_COCIT_404692 are exactly identical! WARNING: Sequences tr_B2W1H9_B2W1H9_PYRTR_426418 and tr_A0A2W1D7S1_A0A2W1D7S1_9PLEO_45151 are exactly identical! WARNING: Sequences tr_B8N9X0_B8N9X0_ASPFN_332952 and tr_A0A2G7G683_A0A2G7G683_9EURO_656916 are exactly identical! WARNING: Sequences tr_A0A179UFB2_A0A179UFB2_BLAGS_559298 and tr_C5GE48_C5GE48_AJEDR_559297 are exactly identical! WARNING: Sequences tr_F9G3U6_F9G3U6_FUSOF_660025 and tr_A0A0D2XVL9_A0A0D2XVL9_FUSO4_426428 are exactly identical! WARNING: Sequences tr_F9G3U6_F9G3U6_FUSOF_660025 and tr_A0A2H3SZT8_A0A2H3SZT8_FUSOX_5507 are exactly identical! WARNING: Sequences tr_F9G3U6_F9G3U6_FUSOF_660025 and tr_A0A2H3HT67_A0A2H3HT67_FUSOX_327505 are exactly identical! WARNING: Sequences tr_C6H865_C6H865_AJECH_544712 and tr_F0U5I6_F0U5I6_AJEC8_544711 are exactly identical! WARNING: Sequences tr_Q2UGF9_Q2UGF9_ASPOR_510516 and tr_O93828_O93828_ASPOZ_5062 are exactly identical! WARNING: Sequences tr_A0A0E0GBU9_A0A0E0GBU9_ORYNI_4536 and tr_A0A0E0NJT8_A0A0E0NJT8_ORYRU_4529 are exactly identical! WARNING: Sequences tr_A0A0E0GBU9_A0A0E0GBU9_ORYNI_4536 and tr_Q6YVI0_Q6YVI0_ORYSJ_39947 are exactly identical! WARNING: Sequences tr_G7XJK4_G7XJK4_ASPKW_1033177 and tr_A0A146FHM8_A0A146FHM8_9EURO_1069201 are exactly identical! WARNING: Sequences tr_G2YDF6_G2YDF6_BOTF4_999810 and tr_M7U4W6_M7U4W6_BOTF1_1290391 are exactly identical! WARNING: Sequences tr_F2SYF6_F2SYF6_TRIRC_559305 and tr_A0A178F7E1_A0A178F7E1_TRIRU_5551 are exactly identical! WARNING: Sequences tr_U5HD96_U5HD96_USTV1_683840 and tr_A0A2X0MK03_A0A2X0MK03_9BASI_796604 are exactly identical! WARNING: Sequences tr_V2YCH9_V2YCH9_MONRO_1381753 and tr_A0A0W0FIL7_A0A0W0FIL7_9AGAR_221103 are exactly identical! WARNING: Sequences tr_W2QI29_W2QI29_PHYPN_761204 and tr_W2HB06_W2HB06_PHYPR_4792 are exactly identical! WARNING: Sequences tr_A0A015MFG4_A0A015MFG4_9GLOM_1432141 and tr_A0A2I1GHJ4_A0A2I1GHJ4_9GLOM_588596 are exactly identical! WARNING: Sequences tr_A0A015MFG4_A0A015MFG4_9GLOM_1432141 and tr_A0A2H5UA25_A0A2H5UA25_RHIID_747089 are exactly identical! WARNING: Sequences tr_A0A096N4P9_A0A096N4P9_PAPAN_9555 and tr_A0A0D9RNI9_A0A0D9RNI9_CHLSB_60711 are exactly identical! WARNING: Sequences tr_A0A094EK97_A0A094EK97_9PEZI_1420912 and tr_A0A1B8GLP2_A0A1B8GLP2_9PEZI_342668 are exactly identical! WARNING: Sequences tr_A0A0F8X8T5_A0A0F8X8T5_9EURO_308745 and tr_A0A2T5LM01_A0A2T5LM01_9EURO_1392256 are exactly identical! WARNING: Sequences tr_A0A0A1MYR9_A0A0A1MYR9_9FUNG_58291 and tr_A0A367JMZ9_A0A367JMZ9_9FUNG_86630 are exactly identical! WARNING: Sequences tr_A0A101MRH9_A0A101MRH9_9EURO_48697 and tr_A0A1V6R0U5_A0A1V6R0U5_9EURO_60172 are exactly identical! WARNING: Sequences tr_A0A1S3ZHV9_A0A1S3ZHV9_TOBAC_4097 and tr_A0A1U7Y202_A0A1U7Y202_NICSY_4096 are exactly identical! WARNING: Duplicate sequences found: 25 NOTE: Reduced alignment (with duplicates and gap-only sites/taxa removed) NOTE: was saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P00480/4_raxmlng_ancestral/P00480.raxml.reduced.phy Alignment comprises 1 partitions and 354 sites Partition 0: noname Model: LG4X+R4 Alignment sites: 354 Gaps: 12.17 % Invariant sites: 0.00 % NOTE: Binary MSA file created: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P00480/4_raxmlng_ancestral/P00480.raxml.rba Parallelization scheme autoconfig: 1 worker(s) x 4 thread(s) Parallel reduction/worker buffer size: 1 KB / 0 KB [00:00:00] Loading user starting tree(s) from: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P00480/3_mltree/P00480.raxml.bestTree_unrooted [00:00:00] Data distribution: max. partitions/sites/weight per thread: 1 / 89 / 7120 [00:00:00] Data distribution: max. searches per worker: 1 Starting ML tree search with 1 distinct starting trees [00:00:00] Tree #1, initial LogLikelihood: -130698.778820 [00:00:00 -130698.778820] Initial branch length optimization [00:00:00 -130460.416340] Model parameter optimization (eps = 0.100000) [00:00:20] Tree #1, final logLikelihood: -130083.634655 Optimized model parameters: Partition 0: noname Rate heterogeneity: FREE (4 cats, mean), weights&rates: (0.229398,0.288894) (0.285550,0.386789) (0.247283,1.035427) (0.237769,2.385665) Base frequencies (model): M0: 0.147383 0.017579 0.058208 0.017707 0.026331 0.041582 0.017494 0.027859 0.011849 0.076971 0.147823 0.019535 0.037132 0.029940 0.008059 0.088179 0.089653 0.006477 0.032308 0.097931 M1: 0.063139 0.066357 0.011586 0.066571 0.010800 0.009276 0.053984 0.146986 0.034214 0.088822 0.098196 0.032390 0.021263 0.072697 0.016761 0.020711 0.020797 0.025463 0.045615 0.094372 M2: 0.062457 0.066826 0.049332 0.065270 0.006513 0.041231 0.058965 0.080852 0.028024 0.037024 0.075925 0.064131 0.019620 0.028710 0.104579 0.056388 0.062027 0.008241 0.033124 0.050760 M3: 0.106471 0.074171 0.044513 0.096390 0.002148 0.066733 0.158908 0.037625 0.020691 0.014608 0.028797 0.105352 0.007864 0.007477 0.083595 0.055726 0.047711 0.003975 0.010088 0.027159 Substitution rates (model): M 0: 0.295719 0.067388 0.253712 1.029289 0.107964 0.514644 10.868848 0.380498 0.084223 0.086976 0.188789 0.286389 0.155567 1.671061 2.132922 0.529591 0.115551 0.102453 0.916683 0.448317 0.457483 0.576016 1.741924 0.736017 0.704334 5.658311 0.123387 0.221777 93.433377 0.382175 0.235965 6.535048 0.525521 0.303537 0.641259 0.289466 0.102065 2.358429 0.251987 0.216561 0.503084 0.435271 4.873453 0.090748 0.033310 0.746537 0.128905 0.127321 0.904011 0.939733 0.435450 0.046646 0.262076 0.043986 0.189008 0.599450 109.901504 1.070052 5.229858 0.052764 0.021407 0.621146 0.081091 0.205164 5.164456 0.747330 0.308078 0.260889 0.185083 0.080708 0.029955 0.084794 1.862626 0.553477 0.151733 0.230320 0.096955 0.352526 0.590018 0.386853 1.559564 0.606648 0.587531 0.592318 0.885230 4.117654 0.246260 6.508329 0.054187 0.195703 1.669092 0.810168 0.066081 2.437439 0.165666 0.106333 0.093417 0.035149 0.072549 1.202023 1.634845 0.060194 0.069359 2.448827 0.232297 0.064822 3.537387 0.435384 0.290413 0.280695 0.105999 0.206603 0.404968 0.048984 0.069963 0.256662 0.228519 0.241077 4.320442 3.656545 0.290216 0.307466 0.096556 0.306067 0.204296 0.504221 1.991533 0.655465 6.799829 11.291065 0.961142 0.448965 6.227274 20.304886 0.205944 1.495537 0.091940 1.994320 0.754940 0.170343 0.050315 0.372166 0.206332 0.097050 5.381403 0.122332 3.256485 2.261319 0.848067 0.064441 0.102493 0.459041 0.133091 0.561215 0.457430 0.163849 5.260446 0.360946 0.389413 0.033291 0.115301 0.112593 1.559944 0.426508 0.132547 0.498634 0.559069 0.264728 0.693307 0.438856 0.306683 0.109129 18.392863 66.647302 0.400021 4.586081 2.099355 0.411347 0.476350 0.584622 3.634276 0.101797 0.148995 0.089177 0.034710 0.063603 0.755865 20.561407 0.133790 0.154902 M 1: 0.066142 0.590377 0.069930 9.850951 1.101363 0.150375 0.568586 0.051668 0.127170 0.292429 0.071458 1.218562 0.075144 7.169085 30.139501 13.461692 0.021372 0.045779 4.270235 0.468325 0.013688 0.302287 1.353957 0.028386 0.037750 0.262130 0.016923 0.064289 0.855973 0.079621 0.011169 0.161937 0.276530 0.161053 0.081472 0.036742 0.030342 2.851667 3.932151 8.159169 0.219934 0.421974 2.468752 0.344765 0.210724 1.172204 0.763553 0.082464 0.726566 11.149790 4.782635 0.058046 0.498072 0.258487 0.146882 0.249672 0.560142 0.046719 0.106259 0.003656 0.004200 0.014189 0.009876 0.002656 0.040244 0.267322 0.053740 0.006597 0.027639 0.012745 0.582670 0.005035 0.275844 0.098208 0.445038 1.217010 0.033969 1.988516 0.681161 0.825960 18.762977 11.949233 0.286794 0.534219 4.336817 3.054085 0.129551 4.210126 0.165753 1.088704 1.889645 3.344809 0.111063 2.067758 3.547017 2.466507 0.188236 0.203493 0.281953 0.037250 0.029788 0.008541 0.014768 0.125869 0.056702 0.004186 0.110993 0.201148 0.139705 0.009201 0.012095 0.043812 0.013513 0.002533 0.005848 0.031390 0.021612 0.004854 0.129497 0.976631 0.053397 0.019475 0.004964 0.015539 0.031779 0.064558 0.065585 0.079927 0.095591 0.196886 0.408834 0.126088 0.037226 0.452302 0.016212 7.278994 0.029917 7.918203 0.450964 0.169797 0.104288 1.578530 0.015909 0.094365 16.179952 0.042762 14.799537 1.506485 0.637893 0.123793 0.641351 0.154810 0.140750 3.416059 0.259400 0.009457 0.090576 0.292108 0.297913 0.017172 0.021976 0.032578 1.375871 0.457399 0.598048 4.418398 0.239749 0.168432 2.950318 0.143327 0.328689 0.125011 0.562720 1.414883 0.227807 3.478333 2.984862 0.061299 0.077470 1.050562 13.974326 0.154326 0.224675 0.112000 0.060703 0.123480 5.294490 0.447011 0.033381 0.045528 M 2: 0.733336 0.558955 0.503360 4.149599 1.415369 1.367574 1.263002 0.994098 0.517204 0.775054 0.763094 1.890137 0.540460 0.200122 4.972745 1.825593 0.450842 0.526135 3.839269 0.597671 0.058964 2.863355 2.872594 0.258365 0.366868 2.578946 0.358350 0.672023 5.349861 0.691594 0.063347 0.032875 0.821562 0.580847 0.661866 0.265730 0.395134 5.581680 1.279881 1.335650 0.397108 1.840061 5.739035 0.284730 0.109781 1.612642 0.466979 0.141582 0.019509 4.670980 1.967383 0.088064 0.581928 0.145401 0.225860 0.434096 2.292917 1.024707 0.821921 0.027824 0.021443 0.088850 0.060820 0.018288 0.042687 1.199607 0.420710 0.037642 0.141233 0.090101 1.043232 0.209978 0.823594 3.039380 1.463390 1.983693 0.397640 2.831098 4.102068 0.059723 5.901348 2.034980 2.600668 5.413080 4.193725 4.534772 0.377181 4.877840 0.370939 1.298542 3.509873 2.646440 0.087872 0.072299 1.139018 0.864479 0.390688 0.322761 0.625409 0.496780 0.532488 0.232460 0.169219 0.755219 0.379926 0.020447 0.023282 0.503875 0.577513 0.109318 0.153776 0.696533 0.398817 0.008940 0.043707 0.436013 0.087640 0.064863 0.036426 1.673207 0.124068 0.218118 0.039217 0.104335 0.349195 0.838324 0.888693 0.488389 1.385133 0.050226 0.962470 0.502294 1.065585 8.351808 0.377304 5.102837 0.561690 7.010411 3.054968 0.039318 0.204155 2.653232 0.564368 0.854294 15.559906 0.401070 8.929538 5.525874 0.067505 0.273372 0.437116 1.927515 0.940458 2.508169 1.357738 0.043394 0.023126 0.567639 1.048288 0.120994 0.180650 0.449074 3.135353 0.012695 0.570771 2.319555 1.856122 0.975427 3.404087 0.015631 0.458799 0.151684 4.154750 11.429924 1.457957 0.233109 0.077004 0.011074 0.026268 0.052132 8.113282 0.377578 0.429221 0.260296 0.222293 0.273138 2.903836 4.731579 0.564762 0.681215 M 3: 0.658412 0.566269 0.854111 0.884454 1.309554 1.272639 1.874713 0.552007 0.227683 0.581512 0.695190 0.967985 0.344015 0.978992 3.427163 2.333253 0.154701 0.221089 2.088785 0.540749 0.058015 5.851132 2.294145 0.182966 0.684164 3.192521 0.528161 1.128882 3.010922 1.012866 0.227296 0.156635 0.878405 0.802754 0.830884 0.431617 0.456530 3.060574 1.279257 1.438430 0.431464 2.075952 4.840271 0.644656 0.266076 2.084975 0.720060 0.291854 0.028961 4.071574 2.258357 0.073037 1.238426 0.199728 0.160296 0.482619 2.992763 1.296206 0.841829 0.031467 0.048542 0.132774 0.133055 0.056045 0.209188 0.925172 0.360522 0.094591 0.313945 0.118104 0.992259 0.086318 2.149634 5.103188 3.775817 3.954021 0.190734 1.776095 4.495841 0.264277 7.063879 2.221150 3.017954 8.558815 4.310199 2.130054 0.571406 4.137385 0.437589 2.071689 2.498630 1.763546 0.116381 0.296578 1.033710 1.283423 0.312579 0.305772 0.681277 0.507160 0.351381 0.189152 0.217780 0.767361 0.278392 0.092075 0.177263 0.451893 0.653836 0.074620 0.181992 0.752277 0.679853 0.025780 0.082005 0.326441 0.343977 0.195877 0.217424 3.057583 0.377558 0.401252 0.072258 0.241015 0.665865 1.266791 0.680174 0.717301 4.001286 0.362942 1.189259 0.964545 1.350568 12.869737 0.531100 8.904999 0.652629 10.091413 2.671718 0.086367 0.359932 4.797423 0.336801 1.021885 23.029406 0.440178 14.013035 5.069337 0.539010 0.742569 0.780580 1.331875 1.531589 4.414850 1.082703 0.091278 0.172734 0.693405 1.422571 0.068958 0.163829 0.481711 4.643214 0.121821 0.584083 4.216178 1.677263 1.575754 5.046403 0.161015 1.531223 0.599244 5.832025 33.873091 1.914768 1.287474 0.444362 0.076328 0.079916 0.466823 5.231362 0.548763 0.831890 0.382271 0.208791 0.307846 3.717971 5.910440 0.282540 0.964421 Marginal ancestral probabilities saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P00480/4_raxmlng_ancestral/P00480.raxml.ancestralProbs Reconstructed ancestral sequences saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P00480/4_raxmlng_ancestral/P00480.raxml.ancestralStates Node-labeled tree saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P00480/4_raxmlng_ancestral/P00480.raxml.ancestralTree Execution log saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/P00480/4_raxmlng_ancestral/P00480.raxml.log Analysis started: 03-Jun-2021 02:07:24 / finished: 03-Jun-2021 02:07:48 Elapsed time: 23.483 seconds Consumed energy: 1.964 Wh