RAxML-NG v. 1.0.2 released on 22.02.2021 by The Exelixis Lab. Developed by: Alexey M. Kozlov and Alexandros Stamatakis. Contributors: Diego Darriba, Tomas Flouri, Benoit Morel, Sarah Lutteropp, Ben Bettisworth. Latest version: https://github.com/amkozlov/raxml-ng Questions/problems/suggestions? Please visit: https://groups.google.com/forum/#!forum/raxml System: Intel(R) Xeon(R) CPU E5-2690 v4 @ 2.60GHz, 28 cores, 251 GB RAM RAxML-NG was called at 03-Jun-2021 02:36:33 as follows: raxml-ng --ancestral --msa /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/O15528/2_msa/O15528_nogap_msa.fasta --tree /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/O15528/3_mltree/O15528.raxml.bestTree_unrooted --model LG4X --prefix /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/O15528/4_raxmlng_ancestral/O15528 --threads 4 Analysis options: run mode: Ancestral state reconstruction start tree(s): user random seed: 1622676993 tip-inner: ON pattern compression: OFF per-rate scalers: OFF site repeats: OFF branch lengths: proportional (ML estimate, algorithm: NR-FAST) SIMD kernels: AVX2 parallelization: coarse-grained (auto), PTHREADS (4 threads), thread pinning: OFF [00:00:00] Reading alignment from file: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/O15528/2_msa/O15528_nogap_msa.fasta [00:00:00] Loaded alignment with 1001 taxa and 508 sites WARNING: Sequences tr_Q29JM1_Q29JM1_DROPS_46245 and tr_B4H385_B4H385_DROPE_7234 are exactly identical! WARNING: Sequences tr_A0A179V205_A0A179V205_BLAGS_559298 and tr_A0A179U3D7_A0A179U3D7_AJEDR_559297 are exactly identical! WARNING: Sequences tr_H2Q6C4_H2Q6C4_PANTR_9598 and tr_A0A2R9ALD4_A0A2R9ALD4_PANPA_9597 are exactly identical! WARNING: Sequences tr_K7CD19_K7CD19_PANTR_9598 and tr_A0A2R9CKX8_A0A2R9CKX8_PANPA_9597 are exactly identical! WARNING: Sequences tr_F6U4F6_F6U4F6_MACMU_9544 and tr_A0A0D9RS29_A0A0D9RS29_CHLSB_60711 are exactly identical! WARNING: Sequences tr_F7H3A6_F7H3A6_MACMU_9544 and tr_A0A2K6BWR1_A0A2K6BWR1_MACNE_9545 are exactly identical! WARNING: Sequences tr_B3RWY4_B3RWY4_TRIAD_10228 and tr_A0A369SCJ7_A0A369SCJ7_9METZ_287889 are exactly identical! WARNING: Sequences tr_G7P937_G7P937_MACFA_9541 and tr_A0A2K5NIM5_A0A2K5NIM5_CERAT_9531 are exactly identical! WARNING: Sequences tr_G7P937_G7P937_MACFA_9541 and tr_A0A2K6DZD8_A0A2K6DZD8_MACNE_9545 are exactly identical! WARNING: Sequences tr_G7P937_G7P937_MACFA_9541 and tr_A0A2K5XZF7_A0A2K5XZF7_MANLE_9568 are exactly identical! WARNING: Sequences tr_G7PLG2_G7PLG2_MACFA_9541 and tr_A0A096NNZ9_A0A096NNZ9_PAPAN_9555 are exactly identical! WARNING: Sequences tr_G7PLG2_G7PLG2_MACFA_9541 and tr_A0A0D9RW85_A0A0D9RW85_CHLSB_60711 are exactly identical! WARNING: Sequences tr_G7PLG2_G7PLG2_MACFA_9541 and tr_A0A2K5LAE5_A0A2K5LAE5_CERAT_9531 are exactly identical! WARNING: Sequences tr_G7PLG2_G7PLG2_MACFA_9541 and tr_A0A2K6DIC2_A0A2K6DIC2_MACNE_9545 are exactly identical! WARNING: Sequences tr_A0A0V0SML7_A0A0V0SML7_9BILA_6336 and tr_A0A0V1A4K6_A0A0V1A4K6_9BILA_990121 are exactly identical! WARNING: Sequences tr_A0A0V1CUR6_A0A0V1CUR6_TRIBR_45882 and tr_A0A0V0W725_A0A0V0W725_9BILA_92179 are exactly identical! WARNING: Sequences tr_A0A0V1CUR6_A0A0V1CUR6_TRIBR_45882 and tr_A0A0V0VTM1_A0A0V0VTM1_9BILA_181606 are exactly identical! WARNING: Sequences tr_A0A0V1CUR6_A0A0V1CUR6_TRIBR_45882 and tr_A0A0V1L9X3_A0A0V1L9X3_9BILA_6335 are exactly identical! WARNING: Sequences tr_A0A0V1CUR6_A0A0V1CUR6_TRIBR_45882 and tr_A0A0V1P5H7_A0A0V1P5H7_9BILA_92180 are exactly identical! WARNING: Sequences tr_A0A0V1CUR6_A0A0V1CUR6_TRIBR_45882 and tr_A0A0V0U3T1_A0A0V0U3T1_9BILA_144512 are exactly identical! WARNING: Sequences tr_A0A164N9T1_A0A164N9T1_9HOMO_1314777 and tr_A0A166D5D0_A0A166D5D0_9HOMO_1314776 are exactly identical! WARNING: Sequences tr_A0A1U7Q3G1_A0A1U7Q3G1_MESAU_10036 and sp_Q9EPT4_CP11A_MESAU_10036 are exactly identical! WARNING: Sequences tr_A0A226NP36_A0A226NP36_CALSU_9009 and tr_A0A226PCH3_A0A226PCH3_COLVI_9014 are exactly identical! WARNING: Sequences tr_A0A2U1LWA4_A0A2U1LWA4_ARTAN_35608 and tr_A0A2U1PC00_A0A2U1PC00_ARTAN_35608 are exactly identical! WARNING: Duplicate sequences found: 24 NOTE: Reduced alignment (with duplicates and gap-only sites/taxa removed) NOTE: was saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/O15528/4_raxmlng_ancestral/O15528.raxml.reduced.phy Alignment comprises 1 partitions and 508 sites Partition 0: noname Model: LG4X+R4 Alignment sites: 508 Gaps: 18.57 % Invariant sites: 0.00 % NOTE: Binary MSA file created: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/O15528/4_raxmlng_ancestral/O15528.raxml.rba Parallelization scheme autoconfig: 1 worker(s) x 4 thread(s) Parallel reduction/worker buffer size: 1 KB / 0 KB [00:00:00] Loading user starting tree(s) from: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/O15528/3_mltree/O15528.raxml.bestTree_unrooted [00:00:00] Data distribution: max. partitions/sites/weight per thread: 1 / 127 / 10160 [00:00:00] Data distribution: max. searches per worker: 1 Starting ML tree search with 1 distinct starting trees [00:00:00] Tree #1, initial LogLikelihood: -330087.493662 [00:00:00 -330087.493662] Initial branch length optimization [00:00:01 -328125.836288] Model parameter optimization (eps = 0.100000) [00:00:36] Tree #1, final logLikelihood: -327718.283930 Optimized model parameters: Partition 0: noname Rate heterogeneity: FREE (4 cats, mean), weights&rates: (0.046442,0.205317) (0.198927,0.402946) (0.383777,0.801208) (0.370854,1.625496) Base frequencies (model): M0: 0.147383 0.017579 0.058208 0.017707 0.026331 0.041582 0.017494 0.027859 0.011849 0.076971 0.147823 0.019535 0.037132 0.029940 0.008059 0.088179 0.089653 0.006477 0.032308 0.097931 M1: 0.063139 0.066357 0.011586 0.066571 0.010800 0.009276 0.053984 0.146986 0.034214 0.088822 0.098196 0.032390 0.021263 0.072697 0.016761 0.020711 0.020797 0.025463 0.045615 0.094372 M2: 0.062457 0.066826 0.049332 0.065270 0.006513 0.041231 0.058965 0.080852 0.028024 0.037024 0.075925 0.064131 0.019620 0.028710 0.104579 0.056388 0.062027 0.008241 0.033124 0.050760 M3: 0.106471 0.074171 0.044513 0.096390 0.002148 0.066733 0.158908 0.037625 0.020691 0.014608 0.028797 0.105352 0.007864 0.007477 0.083595 0.055726 0.047711 0.003975 0.010088 0.027159 Substitution rates (model): M 0: 0.295719 0.067388 0.253712 1.029289 0.107964 0.514644 10.868848 0.380498 0.084223 0.086976 0.188789 0.286389 0.155567 1.671061 2.132922 0.529591 0.115551 0.102453 0.916683 0.448317 0.457483 0.576016 1.741924 0.736017 0.704334 5.658311 0.123387 0.221777 93.433377 0.382175 0.235965 6.535048 0.525521 0.303537 0.641259 0.289466 0.102065 2.358429 0.251987 0.216561 0.503084 0.435271 4.873453 0.090748 0.033310 0.746537 0.128905 0.127321 0.904011 0.939733 0.435450 0.046646 0.262076 0.043986 0.189008 0.599450 109.901504 1.070052 5.229858 0.052764 0.021407 0.621146 0.081091 0.205164 5.164456 0.747330 0.308078 0.260889 0.185083 0.080708 0.029955 0.084794 1.862626 0.553477 0.151733 0.230320 0.096955 0.352526 0.590018 0.386853 1.559564 0.606648 0.587531 0.592318 0.885230 4.117654 0.246260 6.508329 0.054187 0.195703 1.669092 0.810168 0.066081 2.437439 0.165666 0.106333 0.093417 0.035149 0.072549 1.202023 1.634845 0.060194 0.069359 2.448827 0.232297 0.064822 3.537387 0.435384 0.290413 0.280695 0.105999 0.206603 0.404968 0.048984 0.069963 0.256662 0.228519 0.241077 4.320442 3.656545 0.290216 0.307466 0.096556 0.306067 0.204296 0.504221 1.991533 0.655465 6.799829 11.291065 0.961142 0.448965 6.227274 20.304886 0.205944 1.495537 0.091940 1.994320 0.754940 0.170343 0.050315 0.372166 0.206332 0.097050 5.381403 0.122332 3.256485 2.261319 0.848067 0.064441 0.102493 0.459041 0.133091 0.561215 0.457430 0.163849 5.260446 0.360946 0.389413 0.033291 0.115301 0.112593 1.559944 0.426508 0.132547 0.498634 0.559069 0.264728 0.693307 0.438856 0.306683 0.109129 18.392863 66.647302 0.400021 4.586081 2.099355 0.411347 0.476350 0.584622 3.634276 0.101797 0.148995 0.089177 0.034710 0.063603 0.755865 20.561407 0.133790 0.154902 M 1: 0.066142 0.590377 0.069930 9.850951 1.101363 0.150375 0.568586 0.051668 0.127170 0.292429 0.071458 1.218562 0.075144 7.169085 30.139501 13.461692 0.021372 0.045779 4.270235 0.468325 0.013688 0.302287 1.353957 0.028386 0.037750 0.262130 0.016923 0.064289 0.855973 0.079621 0.011169 0.161937 0.276530 0.161053 0.081472 0.036742 0.030342 2.851667 3.932151 8.159169 0.219934 0.421974 2.468752 0.344765 0.210724 1.172204 0.763553 0.082464 0.726566 11.149790 4.782635 0.058046 0.498072 0.258487 0.146882 0.249672 0.560142 0.046719 0.106259 0.003656 0.004200 0.014189 0.009876 0.002656 0.040244 0.267322 0.053740 0.006597 0.027639 0.012745 0.582670 0.005035 0.275844 0.098208 0.445038 1.217010 0.033969 1.988516 0.681161 0.825960 18.762977 11.949233 0.286794 0.534219 4.336817 3.054085 0.129551 4.210126 0.165753 1.088704 1.889645 3.344809 0.111063 2.067758 3.547017 2.466507 0.188236 0.203493 0.281953 0.037250 0.029788 0.008541 0.014768 0.125869 0.056702 0.004186 0.110993 0.201148 0.139705 0.009201 0.012095 0.043812 0.013513 0.002533 0.005848 0.031390 0.021612 0.004854 0.129497 0.976631 0.053397 0.019475 0.004964 0.015539 0.031779 0.064558 0.065585 0.079927 0.095591 0.196886 0.408834 0.126088 0.037226 0.452302 0.016212 7.278994 0.029917 7.918203 0.450964 0.169797 0.104288 1.578530 0.015909 0.094365 16.179952 0.042762 14.799537 1.506485 0.637893 0.123793 0.641351 0.154810 0.140750 3.416059 0.259400 0.009457 0.090576 0.292108 0.297913 0.017172 0.021976 0.032578 1.375871 0.457399 0.598048 4.418398 0.239749 0.168432 2.950318 0.143327 0.328689 0.125011 0.562720 1.414883 0.227807 3.478333 2.984862 0.061299 0.077470 1.050562 13.974326 0.154326 0.224675 0.112000 0.060703 0.123480 5.294490 0.447011 0.033381 0.045528 M 2: 0.733336 0.558955 0.503360 4.149599 1.415369 1.367574 1.263002 0.994098 0.517204 0.775054 0.763094 1.890137 0.540460 0.200122 4.972745 1.825593 0.450842 0.526135 3.839269 0.597671 0.058964 2.863355 2.872594 0.258365 0.366868 2.578946 0.358350 0.672023 5.349861 0.691594 0.063347 0.032875 0.821562 0.580847 0.661866 0.265730 0.395134 5.581680 1.279881 1.335650 0.397108 1.840061 5.739035 0.284730 0.109781 1.612642 0.466979 0.141582 0.019509 4.670980 1.967383 0.088064 0.581928 0.145401 0.225860 0.434096 2.292917 1.024707 0.821921 0.027824 0.021443 0.088850 0.060820 0.018288 0.042687 1.199607 0.420710 0.037642 0.141233 0.090101 1.043232 0.209978 0.823594 3.039380 1.463390 1.983693 0.397640 2.831098 4.102068 0.059723 5.901348 2.034980 2.600668 5.413080 4.193725 4.534772 0.377181 4.877840 0.370939 1.298542 3.509873 2.646440 0.087872 0.072299 1.139018 0.864479 0.390688 0.322761 0.625409 0.496780 0.532488 0.232460 0.169219 0.755219 0.379926 0.020447 0.023282 0.503875 0.577513 0.109318 0.153776 0.696533 0.398817 0.008940 0.043707 0.436013 0.087640 0.064863 0.036426 1.673207 0.124068 0.218118 0.039217 0.104335 0.349195 0.838324 0.888693 0.488389 1.385133 0.050226 0.962470 0.502294 1.065585 8.351808 0.377304 5.102837 0.561690 7.010411 3.054968 0.039318 0.204155 2.653232 0.564368 0.854294 15.559906 0.401070 8.929538 5.525874 0.067505 0.273372 0.437116 1.927515 0.940458 2.508169 1.357738 0.043394 0.023126 0.567639 1.048288 0.120994 0.180650 0.449074 3.135353 0.012695 0.570771 2.319555 1.856122 0.975427 3.404087 0.015631 0.458799 0.151684 4.154750 11.429924 1.457957 0.233109 0.077004 0.011074 0.026268 0.052132 8.113282 0.377578 0.429221 0.260296 0.222293 0.273138 2.903836 4.731579 0.564762 0.681215 M 3: 0.658412 0.566269 0.854111 0.884454 1.309554 1.272639 1.874713 0.552007 0.227683 0.581512 0.695190 0.967985 0.344015 0.978992 3.427163 2.333253 0.154701 0.221089 2.088785 0.540749 0.058015 5.851132 2.294145 0.182966 0.684164 3.192521 0.528161 1.128882 3.010922 1.012866 0.227296 0.156635 0.878405 0.802754 0.830884 0.431617 0.456530 3.060574 1.279257 1.438430 0.431464 2.075952 4.840271 0.644656 0.266076 2.084975 0.720060 0.291854 0.028961 4.071574 2.258357 0.073037 1.238426 0.199728 0.160296 0.482619 2.992763 1.296206 0.841829 0.031467 0.048542 0.132774 0.133055 0.056045 0.209188 0.925172 0.360522 0.094591 0.313945 0.118104 0.992259 0.086318 2.149634 5.103188 3.775817 3.954021 0.190734 1.776095 4.495841 0.264277 7.063879 2.221150 3.017954 8.558815 4.310199 2.130054 0.571406 4.137385 0.437589 2.071689 2.498630 1.763546 0.116381 0.296578 1.033710 1.283423 0.312579 0.305772 0.681277 0.507160 0.351381 0.189152 0.217780 0.767361 0.278392 0.092075 0.177263 0.451893 0.653836 0.074620 0.181992 0.752277 0.679853 0.025780 0.082005 0.326441 0.343977 0.195877 0.217424 3.057583 0.377558 0.401252 0.072258 0.241015 0.665865 1.266791 0.680174 0.717301 4.001286 0.362942 1.189259 0.964545 1.350568 12.869737 0.531100 8.904999 0.652629 10.091413 2.671718 0.086367 0.359932 4.797423 0.336801 1.021885 23.029406 0.440178 14.013035 5.069337 0.539010 0.742569 0.780580 1.331875 1.531589 4.414850 1.082703 0.091278 0.172734 0.693405 1.422571 0.068958 0.163829 0.481711 4.643214 0.121821 0.584083 4.216178 1.677263 1.575754 5.046403 0.161015 1.531223 0.599244 5.832025 33.873091 1.914768 1.287474 0.444362 0.076328 0.079916 0.466823 5.231362 0.548763 0.831890 0.382271 0.208791 0.307846 3.717971 5.910440 0.282540 0.964421 Marginal ancestral probabilities saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/O15528/4_raxmlng_ancestral/O15528.raxml.ancestralProbs Reconstructed ancestral sequences saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/O15528/4_raxmlng_ancestral/O15528.raxml.ancestralStates Node-labeled tree saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/O15528/4_raxmlng_ancestral/O15528.raxml.ancestralTree Execution log saved to: /truba/home/emrah/WORKFOLDER/PROD/020621_run/phylogeny-snakemake/results/O15528/4_raxmlng_ancestral/O15528.raxml.log Analysis started: 03-Jun-2021 02:36:33 / finished: 03-Jun-2021 02:37:15 Elapsed time: 41.171 seconds Consumed energy: 2.822 Wh